Journal: Biomolecules

Article Title: Pterostilbene Enhances Cytotoxicity and Chemosensitivity in Human Pancreatic Cancer Cells

doi: 10.3390/biom10050709

Figure Lengend Snippet: Effect of pterostilbene on autophagy-related protein expression in MIA PaCa-2 cells and MIA PaCa-2 GEMR cells. Cells were treated with 50 and 75 μM pterostilbene for 72 h, and the expression levels of Atg5, Beclin-1 and LC3 II protein in ( A ) MIA PaCa-2 and ( E ) MIA PaCa-2 GEMR cells were analyzed by western blotting. The relative expression levels of ( B , F ) Atg5, ( C , G ) Beclin-1, and ( D , H ) LC3 II are expressed as the mean ± SD ( n = 3). p values were considered statistically significant when * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the untreated control.

Article Snippet: Microtubule-associated protein light chain 3 II (LC3 II) antibody, peroxidase goat anti-mouse and peroxidase goat anti-rabbit were purchased from GeneTex (Irving, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Oncology Reports

Article Title: β2-adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy

doi: 10.3892/or.2019.7266

Figure Lengend Snippet: Correlation of β1-AR with autophagic markers.

Article Snippet: LC3-II (cat. no. A11923) for IHC staining was purchased from Abclonal.

Techniques:

Journal: Oncology Reports

Article Title: β2-adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy

doi: 10.3892/or.2019.7266

Figure Lengend Snippet: Correlation of β2-AR with autophagic markers.

Article Snippet: LC3-II (cat. no. A11923) for IHC staining was purchased from Abclonal.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of A β and Autophagy

doi: 10.1155/2020/6807879

Figure Lengend Snippet: The expression of Beclin-1 and LC3-II in the hippocampal neurons. Representative immunofluorescence images showing in situ expression of Beclin-1 (a) and LC3-II (b) in the differentially treated hippocampal neurons (magnification: ×100). Scale bar = 100 μ m. Quantification of Beclin-1 (c) and LC3-II (d) in the hippocampal neurons in each group (% of Control). Data are presented as mean ± SD. ## P < 0.01, vs control group; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs model group.

Article Snippet: The cells were then incubated overnight with anti-Beclin-1 (1 : 200; ImmunoWay, USA) and anti-LC3-II (1 : 200; ImmunoWay, USA) antibodies at 4°C.

Techniques: Expressing, Immunofluorescence, In Situ

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of A β and Autophagy

doi: 10.1155/2020/6807879

Figure Lengend Snippet: The expression levels of Beclin-1, LC3-II, BACE1, PS-1, and ADAM17 in hippocampal neurons. Data are presented as mean ± SD. ## P < 0.01, vs control group; ∗∗ P < 0.01, ∗∗∗ P < 0.001, vs model group.

Article Snippet: The cells were then incubated overnight with anti-Beclin-1 (1 : 200; ImmunoWay, USA) and anti-LC3-II (1 : 200; ImmunoWay, USA) antibodies at 4°C.

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Yuan-Zhi Decoction and Its Active Ingredients in Both In Vivo and In Vitro Models of Chronic Cerebral Hypoperfusion by Regulating the Levels of A β and Autophagy

doi: 10.1155/2020/6807879

Figure Lengend Snippet: Correlation analysis between A β 1-40/A β 1-42 and the autophagy-related (Beclin-1 and LC3-II) and APP secretases-related proteins (BACE1, PS-1, and ADAM17) levels in hippocampal neurons. R 2 is the correlation coefficient.

Article Snippet: The cells were then incubated overnight with anti-Beclin-1 (1 : 200; ImmunoWay, USA) and anti-LC3-II (1 : 200; ImmunoWay, USA) antibodies at 4°C.

Techniques: